The fundamental formula for converting RPM to RCF is as follows:
RCF = (RPM² × 1.118 × 10^-5) × r
where:
Term | Definition |
---|---|
RPM | Revolutions per minute, a measure of the speed of the centrifuge rotor |
RCF | Relative centrifugal force, the effective force exerted on a sample within a centrifuge |
g | The acceleration due to gravity, used as a reference for RCF |
Consider a scenario where you have a centrifuge rotor with a radius of 10 cm spinning at 3,000 RPM. Using the formula provided, you can calculate the RCF as follows:
RCF = (3000² × 1.118 × 10^-5) × 10 = 1,007 g
This calculation shows that the sample experiences forces over a thousand times the force of gravity, illustrating the power of centrifugation in laboratory settings.